Journal: Cell Reports

Article Title: Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells

doi: 10.1016/j.celrep.2019.12.057

Figure Lengend Snippet:

Article Snippet: RNA-seq libraries were then prepared from 200 ng of RNA using the NEBNext Ultra II Directional RNA-seq kit (NEB).

Techniques: Plasmid Preparation, Recombinant, Capture-C, Hybridization, Multiplex Assay, Ligation, Software

Journal: Developmental cell

Article Title: ESRP1 mutations cause hearing loss due to defects in alternative splicing that disrupt cochlear development

doi: 10.1016/j.devcel.2017.09.026

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Total RNA (200ng) was used for poly A selected RNA-seq library preparation using the NEBNext® Ultra ™ Directional RNA Library Prep Kit from Illumina® (mRNA) (New England Biolabs) (products: NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490S) and NEBNext® Ultra ™ Directional RNA Library Prep Kit for Illumina® (E7420S).

Techniques: Transduction, Recombinant, Isolation, Derivative Assay, Modification, Sequencing, CRISPR, Software

Journal: Molecular Systems Biology

Article Title: A solid‐phase transfection platform for arrayed CRISPR screens

doi: 10.15252/msb.20198983

Figure Lengend Snippet:

Article Snippet: Tumor pieces were dissociated into single cell suspensions with the human tumor dissociation kit following the manufacturer's instructions (Miltenyi Biotec, Germany).

Techniques: Recombinant, Virus, Sequencing, Mutagenesis, Lysis, Software, Microscopy, Western Blot, Fluorescence